Method for the detection of molecules containing nucleotidic...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) C07H 21/00 (2006.01) G01N 33/53 (2006.01)

Patent

CA 2171469

Method for the detection and/or location of mutations or deletions in nucleotidic sequences by the formation of heteroduplexes between two double strand DNA types capable of generating mismatches at the mutation or deletion sites. The heteroduplexes are revealed by marking of each type of strand using different fluorescent molecules or are screened by passing over a support which specifically retains them.

Procédé de détection et/ou de localisation de mutations ou de délétions dans des séquences par création d'hétéroduplex entre deux types d'ADN double brin susceptibles de former des mésappariements aux sites desdites mutations ou délétions. Les hétéroduplex sont mis en évidence du fait du marquage sur chaque type de brin par des molécules fluorescentes sont criblés par passage sur un support le retenant spécifiquement.

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