Method for the detection of nucleic acids

Details Method for the detection of nucleic acids Method for the detection of nucleic acids

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2467610

A method is described for the specific detection of nucleic acids on a solid phase. The method involves the steps of: A) providing a surface having immobilized nucleic acids or nucleic-acid analogues, which are suitable for forming non-covalent (base-pair) bonds with the target nucleic acids, B) non-stringent hybridization of the target nucleic acids to be detected onto the immobilized nucleic acids from a solution of the analyte nucleic acid, C) labeling of the nucleic acids of the analysis mixture with labeling elements, D) optionally repeated treatment of the surface with a washing liquid in order to remove weakly bound nucleic acids, and E) detection of the nucleic-acid pairs remaining on the surface with the aid of the labeling unit bonded to them, by means of optical, optical- spectroscopic, electrical, mechanical or magnetic detection methods; wherein steps B) and C) can be carried out in any order.

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