G - Physics – 01 – N
Patent
G - Physics
01
N
150/11, 150/3
G01N 33/49 (2006.01) G01N 33/50 (2006.01) G01N 1/30 (2006.01)
Patent
CA 1317533
ABSTRACT OF THE DISCLOSURE This invention relates to a method and composition for the rapid determination of a differential white blood cell count in a sample, which method comprises: a) preparing a reagent solution comprising formaldehyde or paraformaldehyde; a surfactant; a sugar or sugar alcohol; and a buffer; and b) rapidly mixing said reagent solution with the sample to be analyzed to form a reaction mixture, wherein both said reagent solution and said sample are initially at room temperature; and c) rapidly heating said reaction mixture to a temperature of from about 62°C to about 72°C in order to lyse the red blood cells in said sample and fix the white blood cells in said sample.
517378
Cremins John F.
Kim Young Ran
Malin Michael J.
Sclafani Louis D. IV
Mcfadden Fincham
Technicon Instruments Corporation
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