G - Physics – 01 – N
Patent
G - Physics
01
N
167/45
G01N 33/58 (2006.01) C12Q 1/00 (2006.01) G01N 33/54 (1980.01)
Patent
CA 1144069
METHOD FOR THE DETERMINATION OF ANTIGENS AND ANTIBODIES USING SITE-DEACTIVATING MEDIA Abstract of the Disclosure An improved immunoassay sample determi- nation process for determining the presence of a component of an antigen-antibody reaction in a sample is disclosed which substantially eliminates non-specific interactions between the sample and the reaction vessel wall surfaces during the antigen-antibody reaction, to thereby greatly increase the accuracy of the process. In prac- tice, a site-deactivating medium such as an animal- or vegetable-derived total biological fluid or extract (e.g., plasma or gelatin) is covalently bound to the vessel wall surfaces for deactivation purposes. In preferred forms the process is solid-state, wherein one of the components of the antigen-antibody reaction is coupled covalently to the coating medium, and a reaction mixture frac- tion including the sample being determined and the other component which has been labeled, for in- stance by means of a colorimetrically active enzyme. (Docket No. 16737)
363889
Enzyme Technology Company A. Kansas Limited Partnership
Gowling Lafleur Henderson Llp
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