Method for the determination of fibrin split products and...

Details Method for the determination of fibrin split products and... Method for the determination of fibrin split products and...

G - Physics

01

N

150/11

G01N 21/01 (2006.01) G01N 33/48 (2006.01)

Patent

CA 1067379

ABSTRACT OF THE DISCLOSURE A method for the determination of fibrin split products and fibri- nogen split products in blood serum which comprises: A. Adding a specified amount of an imidazole buffer to each well of a microtiter plate; B. Adding an amount equivalent to the amount of (A) of blood serum test sample to the first well of the microtiter plate of (A) and serially diluting, using a microtiter diluter; C. Adding an amount equivalent to the amount of (A), of a dyed Staphlococcus aureus cell suspension in the buffer of (A) to each well of the microtiter plate of (B); and D. Observing the wells of (C) for visi- ble clumping of the Staphylococcus aureus cells as a positive indication of the presence of fibrin split products and fibrinogen split products in the test blood serum. Coloration of the cells greatly improves visualization of the end point of the test. The concentration of fibrinogen split products and/or fibrin split products in the blood serum test sample can be calculated if a comparison test is run with a serum containing a known amount of fibrin- ogen split products and/or fibrin split products.

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