Method for the determination of lactic acid in organic...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/32 (2006.01) C12M 1/34 (2006.01) C12M 1/40 (2006.01) C12Q 1/00 (2006.01) C12Q 1/26 (2006.01) C12Q 1/28 (2006.01) G01N 27/30 (2006.01) G01N 27/327 (2006.01)

Patent

CA 2176632

A method is described for the determination of lactic acid in organic materials of alimentary interest that involves reacting, in a buffered aqueous medium, a sample of the said materials, or a solution obtained by extracting them, with an enzyme system comprising L(+) lactate oxidase, D(-) lactate dehydrogenase and horseradish peroxidase and measuring the concentration of any oxygen produced as a result of the oxidation of the lactic acid contained in the said sample with an amperom- etric electrode that is selective for oxygen. A biosensor that can be used to put the above method into effect is also described.

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