Method for the determination of lupus anticoagulant...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/56 (2006.01) G01N 33/86 (2006.01) G01N 33/92 (2006.01)

Patent

CA 2105606

In order to determine lupus anticoagulant (LA) antibodies in blood, plasma or tissue samples by means of the inhibitory effect of these antibodies on the neutralizing effect of protein C on the blood coagulation system, a defined amount of activated protein C is added to the sample, after incubation the remaining amount of a physiological substrate of protein C or protein S activity in the sample is determined according to known methods and the amount of LA antibodies present is calculated by comparison with a standard containing no LA antibodies. The method according to the present invention can be used to diagnose a predisposition to thrombotic events or disease-dependent risk of thrombosis as well as to monitor a therapy.

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