G - Physics – 01 – N
Patent
G - Physics
01
N
G01N 33/577 (2006.01) C07K 16/24 (2006.01) G01N 33/564 (2006.01) G01N 33/68 (2006.01) G01N 33/88 (2006.01) G01N 33/92 (2006.01)
Patent
CA 2097417
2097417 9307493 PCTABS00021 The method for detecting the sulfidoleukotrienes sLT of the group LTC4, LTD4 and LTE4 by a single immunoenzymatic ELISA assay is based on the interaction between one or several monoclonal anti-sLT antibodies and an sLT conjugated to a revealing enzyme. A biological sample, wherein a content of sLT is assumed, is contacted with a monoclonal anti-sLT antibody bound to a carrier. After the sLTs present in the sample are bound to the said antibody, a conjugate of an sLT with a revealing enzyme is added to the charged carrier. Finally the amount of the conjugate bound to the carrier is evaluated, which amount is in an inverse correlation with the sLT bound to the carrier. The method is useful for in vitro-tests for the diagnosis of inflammatory diseases, as rheumatic diseases, immunodeficiencies, allergies or pseudo-allergies. With a priming of the biological samples with cytokines, e.g. with IL3, IL5 or GM-CSF as priming agents, the sensitivity of the method can be increased. The assay is sufficiently sensitive to detect sLT produced by a few reactive cells in minute volumes of whole blood.
de Weck Alain Ladislas
Robic
LandOfFree
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