G - Physics – 01 – N
Patent
G - Physics
01
N
G01N 33/577 (2006.01) G01N 33/543 (2006.01) G01N 33/58 (2006.01) G01N 33/68 (2006.01) G01N 33/78 (2006.01)
Patent
CA 2132830
2132830 9322675 PCTABS00027 Method for the determination of the amount of a ligand (L), in particular of a free ligand in the presence of physiological binding proteins, in a sample of a biological fluid, in which the biological fluid containing the ligand (L) to be determined is incubated in the presence of a dissolved ligand derivative (L-IgG), which can be converted into an insoluble form, in the liquid phase with less than the stoichiometric amount of a labelled specific binder (Ak*) which binds both the ligand (L) to be determined and the ligand derivative (L-IgG) so that the ligand (L) to be determined and the ligand derivative (L-IgG) compete for the labelled specific binder (Ak*), and in which the ligand derivative (L-IgG) is converted into an insoluble form and, after separation of the insoluble components of the assay system from those components of the assay system which remain in the liquid phase, the amount of the ligand (L) to be determined in the biological fluid is isolated from the amount of the labelled specific binder (Ak*) bound to the ligand derivative (L-IgG), wherein, according to the invention, a conjugate of the ligand with a substrate protein is used as the ligand derivative (L-IgG) and the reaction is carried out in a test tube whose walls are coated with a protein material (anti-IgG) which specifically binds the substrate protein part of the ligand derivative (L-IgG) without affecting the bond between the ligand part of the conjugate and the specific binder (Ak*).
Bergmann Andreas
Struck Joachim
Henning Berlin Gmbh
Osler Hoskin & Harcourt Llp
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