C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
150/16.1
C12N 1/00 (2006.01) G01N 33/02 (2006.01) G01N 33/04 (2006.01) G01N 33/12 (2006.01)
Patent
CA 1040517
ABSTRACT OF THE DISCLOSURE A method for the rapid determination of the presence or absence of residues of antibiotic, for example penicillin, in liquids, (for example milk) and meat, which comprises introducing spores of a microorganism, which possesses a high sensitivity for the antibiotic to be determined, in an agar medium in such a manner that the spores are prevented from starting to germ by lack of nutrients, but stay alive, and being sufficiently heavily inoculated that after addition of nutrients and incubation at or near optimal temperature in a short time sufficient growth has proceeded that either growth is observable visually or is indicated by an indicator added to the agar medium, the said spore culture being allowed to solidify in upright test vessels having a cross-sectional dimension sufficiently small to require as little of the agar medium as possible but sufficiently large to enable a correct determination of inhibition of growth of the microorganism to be made, and having a height so that the vessel can contain a sufficient amount of medium and a sufficient amount of sample of liquid to be tested, placing on the agar surface nutrients required for growth, followed, if desired after a pre-incubation period, by adding a predetermined amount of sample liquid to be tested to the test vessel, and incubating at or near optimal temperature the contents of the test vessel for a predetermined time so that the extent of growth or inhibition of growth of the microorganism read in a vertical sense, indicates absence or presence of the antibiotic.
201293
Lameris Sophia A.
Oostendorp Joannes G.
Van Os Jan L.
Gist - Brocades N.v.
Na
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