Method for the diagnosis or the screening of an arbovirus...

C - Chemistry – Metallurgy – 07 – K

Patent

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C07K 19/00 (2006.01) C07K 14/005 (2006.01) C07K 14/18 (2006.01) C12N 9/16 (2006.01) C12N 15/62 (2006.01) C12Q 1/42 (2006.01) G01N 33/53 (2006.01) G01N 33/564 (2006.01) G01N 33/569 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2690764

Method for the diagnosis or the screening of an arbovirus infection and preferably a flaviviridae infection and more preterably a flavivirus infection, reagents useful in said method and their applications. Said method comprises: (i) contacting a sample from the subject or animal with a solid support sensitized with an Ig binding protein which is directed against a specific class of Ig molecules of the subject or animal species under consideration and (ii) incubating the immunocomplex formed in (i) with a detector molecule consisting of a hybrid protein comprising at least an arboviral ED3 domain and an alkaline phosphatase (PhoA), the detection of said immunocomplex being the sign of the presence of an arbovirus in said sample.

L'invention porte sur un procédé de diagnostic ou de criblage d'une infection par arbovirus et, de préférence, une infection par flaviviridae et, de façon davantage préférée, une infection par flavivirus, sur des réactifs utiles dans le procédé et sur leurs applications. Le procédé consiste à (i) mettre en contact un échantillon prélevé sur sujet ou sur animal et présentant un support solide sensibilisé par une protéine de liaison à Ig dirigée contre une classe spécifique de molécules d'Ig du sujet ou de l'espèce animale considéré ; (ii) faire incuber l'immunocomplexe formé en (i) avec une molécule détecteur constituée d'une protéine hybride comprenant au moins un domaine ED3 arboviral et d'une phosphatase alcaline (PhoA), la détection de l'immunocomplexe étant le signe de la présence d'un arbovirus dans l'échantillon.

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