Method for the differentiation of human adult stem cells...

Details Method for the differentiation of human adult stem cells... Method for the differentiation of human adult stem cells...

C - Chemistry, Metallurgy

12

N

C12N 5/071 (2010.01) C12N 5/074 (2010.01) A61K 35/39 (2006.01)

Patent

CA 2701014

Disclosed is a method for differentiating human adult stem cells into insulin-secreting cells. Human adult stem cells, isolated from the subcutaneous adipose tissues around the eyes, are induced to differentiate into insulin-secreting cells in a medium in the presence of cytokines and growth factors including B27 supplement, fibroblast growth factor-2, epidermal growth factor, nicotinamide, glucagon-like peptide- 1, activin A, insulin-like growth factor, betacellulin, etc., with a glucose shift from a high concentration to a low concentration. Having the ability to producing insulin and C- peptide at high levels, the insulin-secreting cells can be excellent curatives for type 1 diabetes mellitus.

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