Method for the enzymatic hydrolysis of chlolesterol esters

C - Chemistry – Metallurgy – 12 – Q

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150/15.4

C12Q 1/60 (2006.01)

Patent

CA 1046389

Abstract of the Disclosure A totally enzymatic method for the hydrolysis of cholesterol esters using a lipase having cholesterol esterase activity and a protease and a method for the quantative determination of total cholesterol in compositions containing both free and esterified cholesterol comprising enzymatically hydrolyzing the cholesterol esters with a lipase having cholesterol esterase activity and A protease and determining total cholesterol by gas-liquid chromatography or some other suitable technique are described.

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