Method for the extraction and analysis of contents made from...

B - Operations – Transporting – 01 – D

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B01D 11/02 (2006.01)

Patent

CA 2464774

The invention relates to a method for the isolation of contents made from organic material, comprising the following method steps: (a) freeze-drying the organic material, (b) extraction of the contents with a polar solvent or solvent mixture (A) and an organic solvent or solvent mixture (B), whereby the extracts from the extractions with (A) and (B) can form one phase, (c) combination of extracts (A) and (B) to give one phase and (d) carrying out an esterification/transesterification in the non-polar phase with an alcohol. The esterification/transesterification is carried out in the presence of a liquid acid. The method is a high-throughput method.

La présente invention concerne un procédé d'isolement de substances à partir d'une matière organique, ce procédé comprenant les étapes suivantes : (a) lyophilisation de la matière organique, (b) extraction des substances au moyen d'un solvant polaire ou d'un mélange de solvants polaires (A) et d'un solvant organique ou d'un mélange de solvants organiques (B), les extraits obtenus au moyen de (A) et (B) pouvant former une phase, (c) combinaison des extraits obtenus au moyen de (A) et (B) pour former une phase et (d) réalisation d'une estérification/transestérification dans la phase non polaire au moyen d'un alcool, cette estérification/transestérification ayant lieu en présence d'un acide volatil. Ce procédé est un procédé à haut rendement.

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