Method for the extraction of periplasmic proteins from...

Details Method for the extraction of periplasmic proteins from... Method for the extraction of periplasmic proteins from...

C - Chemistry, Metallurgy

12

N

C12N 15/11 (2006.01) C07K 1/14 (2006.01) C07K 14/54 (2006.01) C07K 14/61 (2006.01) C12N 1/06 (2006.01) C12N 1/20 (2006.01) C12N 15/67 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2168382

The invention relates to the use of arginine as an agent for the extraction of recombinant periplasmic proteins. The invention further relates to a method for the extraction of a periplasmic protein of interest, which consists in: (1) suspending the pellet of cells or cell debris from cells originating from the culture of a prokaryotic microorganism, transformed by means of an expression vector containing a gene coding for the said protein and means for its expression in the periplasm of the said microorganism, in a buffer solution containing arginine; and (ii) recovering the protein of interest in the supernatant of the bacterial suspension thereby obtained.

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