C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/31 (2006.01) A61K 39/095 (2006.01) C07K 14/01 (2006.01) C07K 14/22 (2006.01) C07K 19/00 (2006.01) C12N 1/21 (2006.01) C12N 15/62 (2006.01) C12N 15/70 (2006.01) A61K 39/00 (2006.01)
Patent
CA 2167677
The present invention relates, in general, to a method for the high level expression of the outer membrane protein meningococcal group B porin proteins and fusion proteins thereof. In particular, the present invention relates to a method of expressing the outer membrane protein meningococcal group B porin proteins in E. coli wherein the meningococcal group B porin proteins and fusion proteins thereof comprise more than 2 % of the total protein expressed in E coli, exemplified by the use of the plasmid expression vector porB-pET-17b depicted in the figure. The invention also relates to a method of purification and refolding of the meningococcal group B porin proteins and fusion proteins thereof and to their use in vaccines.
L'invention concerne, en général, un procédé d'expression à haut niveau des protéines porines du groupe B méningococciques de membrane extérieure ainsi que leurs protéines de fusion. L'invention concerne notamment un procédé d'expression des protéines porines du groupe B méningococciques de membrane extérieure dans E.coli, dans lequel les protéines porines du groupe B méningococciques ainsi que leurs protéines de fusion comprennent plus de 2 % de la protéine totale exprimée dans E.coli, dont on peut citer en exemple l'utilisation du vecteur d'expression de plasmide porB-pET-17b illustré par la figure 1. L'invention concerne également un procédé de purification et de repliement des protéines porines de groupe B méningococciques ainsi que de leurs protéines de fusion, et leur utilisation dans des vaccins.
Blake Milan S.
Hronowski Lucjan J.j.
Liang Shu-Mei
Pullen Jeffrey K.
Qi Huilin L.
Baxter Healthcare S.a.
Mbm Intellectual Property Law Llp
Rockefeller University (the)
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