Method for the identification and/or the quantification of a...

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G01N 33/543 (2006.01) C12Q 1/68 (2006.01) G01N 21/47 (2006.01) G01N 21/86 (2006.01)

Patent

CA 2371658

The present invention is related to a method for the identification and/or the quantification of a target compound obtained from a sample, preferably a biological sample, comprising the steps of: putting into contact the target compound with a capture molecule in order in order to allow a specific binding between said target compound with a capture molecule, said capture molecule being fixed upon a surface of a solid support according to an array comprising a density of at least 20 discrete regions per cm2, each of said discrete regions being fixed with one species of capture molecules, performing a reaction leading to a precipitate formed at the location of said binding, determining the possible presence of precipitate(s) in discrete region(s), and correlating the presence of the precipitate(s) at the discrete region(s) with the identification and/or a quantification of said target compound.

L'invention concerne un procédé permettant l'identification et/ou la quantification d'un composé cible obtenu à partir d'un échantillon, de préférence un échantillon biologique. Ce procédé consiste à mettre le composé cible en contact avec une molécule de capture afin d'établir une liaison spécifique entre ledit composé cible et cette molécule de capture, ladite molécule de capture étant fixée sur la surface d'un support solide suivant une matrice présentant une densité d'au moins 20 régions discrètes par cm?2¿, chacune de ces régions discrètes étant fixée à une espèce de molécules de capture; à produire une réaction aboutissant à un précipité formé à l'emplacement de ladite liaison; à déterminer la présence éventuelle de précipités dans les régions discrètes, et à mettre la présence de précipités dans les régions discrètes en corrélation avec l'identification et/ou la quantification dudit composé cible.

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