Method for the identification of a metabolic pathway family...

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/04 (2006.01)

Patent

CA 2558453

The invention relates to the direct selection of metabolic pathways having a determined function in the transformation of a substrate {Ai} into a target product {B}, which is of interest in the industrial, pharmaceutical or agri-food sectors. More specifically, the invention relates to the detection, within metagenomic libraries, of novel biosynthesis pathways involved in a biochemical reaction having a known product {B}. The selection and characterisation of said novel metabolic pathways enables {B} to be produced enzymatically. The invention provides an alternative to the chemical synthesis of the molecule in question {B}. Moreover, and above all, the invention can be used specifically to target and exploit the only metabolic pathways enabling the transformation of {Ai} into {B}, while eliminating the associated metabolic pathways that can catabolise the target product {B}.

L'invention se rapporte à la sélection directe de voies métaboliques ayant une fonction déterminée dans la transformation d'un substrat {Ai} en un produit cible {B}, présentant un intérêt industriel, pharmaceutique ou agroalimentaire. Plus particulièrement l'invention porte sur la détection, au sein de banques metagénomiques, de nouvelles voies de biosynthèse impliquées dans une réaction biochimique dont on connaît le produit {B). A travers la sélection et la caractérisation de ces nouvelles voies métaboliques, permettant de produire {B} par voie enzymatique, la présente invention offre une alternative à la synthèse chimique de la molécule d'intérêt {B}. Aussi et surtout l'invention permet de cibler et exploiter spécifiquement les seules voies métaboliques permettant la transformation {Ai} en {B}, en s'affranchissant des voies métaboliques connexes susceptibles de cataboliser le produit cible {B}.

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