Method for the identification of enzymes having desired...

Details Method for the identification of enzymes having desired... Method for the identification of enzymes having desired...

C - Chemistry, Metallurgy

12

Q

C12Q 1/02 (2006.01)

Patent

CA 2544076

The invention relates to a method for the identification of enzymes with a desired activity, by random generation of a large collection of enzyme variants, the synthesis of said variants in host organisms, the presentation thereof on the surface of the organisms and the isolation of enzyme variants with the desired properties, by the detection of the covalent deposition of the reaction product on the surface of the host organism.

L'invention concerne un procédé permettant d'identifier des enzymes présentant une activité voulue, par production aléatoire d'une collection importante de variantes d'enzymes, la synthèse de ces variantes dans des organismes hôtes, leur présentation à la surface desdits organismes et l'isolement de variantes d'enzymes aux propriétés voulues, par mise en évidence du dépôt covalent du produit de réaction à la surface de l'organisme hôte.

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