Method for the identification of essential genes and...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/10 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2304369

The present invention relates to a method of identifying essential genes in a genome, based on an insertional mutagenesis of a population of cells or of DNA molecules and subjecting this population of cells or DNA molecules to an amplification process, whereby this total population of cells or DNA molecules which statistically represents at least one full insertionally mutated genome is amplified with at least two primer pairs and the extension products analysed, in order to distinguish essential genes from dispensable genes. The present invention is especially suited to the functional analysis of microbial genomes, and especially to haploid genomes.

La présente invention porte sur un procédé d'identification des gènes essentiels dans un génome, sur la base d'une mutagénèse d'insertion d'une population de cellules ou de molécules d'ADN, ce procédé consistant à soumettre cette population de cellules ou de molécules d'ADN à un processus d'amplification. Toute la population des cellules ou des molécules d'ADN qui représente statistiquement au moins un génome muté par insertion totale est amplifiée avec au moins deux paires d'amorces et les produits d'extension analysés de façon à distinguer les gènes essentiels des gènes pouvant être administrés. Cette invention convient particulièrement à l'analyse fonctionnelle des génomes microbiens, et notamment aux génomes haploïdes.

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