Method for the identification of nucleic acids

C - Chemistry – Metallurgy – 12 – Q

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167/37, 150/8.5

C12Q 1/68 (2006.01) C12N 15/00 (2006.01) G01N 33/58 (2006.01)

Patent

CA 1271705

Abstract hybridization method for the identification of nucleic acids in solution is presented. At least two probes, a detector probe and a capturing probe are used in the method. The detector probe is labeled with a detectable label and to the capturing probe a member of an affinity pair is attached. The capturing probe; target nucleic acid: detector probe hybrid formed in the hybridization reaction is isolated by the aid of the other member of the affinity pair. Suitable affinity pairs are biotin-avidin or biotin-streptavidin homopolynucleotide, antigens-antibodies etc. The method is faster to perform than the traditional sandwich hybridization .

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