Method for the identification of propane-oxidizing bacteria

Details Method for the identification of propane-oxidizing bacteria Method for the identification of propane-oxidizing bacteria

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2691420

The invention relates to a method for the identification of propane -oxidizing bacteria which is based on the identification of at least one fragment of the prmA gene encoding the alpha subunit of the propane monooxygenase enzyme and/or the prmD gene encoding an ancillary protein involved in the oxidation reaction of propane by gene amplification in the presence of pairs of primers selected in correspondence of homologous portions, deduced from the alignment of the prmA and prmD sequences.

L'invention porte sur un procédé d'identification de bactéries d'oxydation du propane, à partir de l'identification d'au moins un fragment du gène prmA codant pour la sous unité alpha de l'enzyme propane mono-oxygénase et/ou du gène prmD codant pour une protéine auxiliaire mise en jeu dans la réaction d'oxydation du propane par une amplification génique en présence de paires d'amorces choisies en correspondance de parties homologues, déduites de l'alignement des séquences prmA et prmD.

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