Method for the immobilization of nucleic acid molecules

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) C07H 21/00 (2006.01) C12N 15/10 (2006.01)

Patent

CA 2155634

An improved method for affecting the immobilization of a synthetic nucleic acid molecule, such as an oligonucleotide, to a solid surface, especially polystyrene or glass. The method is useful in facilitating polymorphic analyses, in hybridization assays, and in solid-phase DNA sequencing.

L'invention se rapporte à un procédé amélioré servant à immobiliser une molécule d'acide nucléique synthétique, telle qu'un oligonucléotide, sur une surface solide, notamment du polystyrène ou du verre. Ce procédé est utile en ce qu'il facilite les analyses polymorphes; il est également utile dans les tests d'hybridation ainsi que dans le séquençage en phase solide de l'ADN.

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