C - Chemistry – Metallurgy – 07 – K
Patent
C - Chemistry, Metallurgy
07
K
C07K 16/18 (2006.01) C07K 16/38 (2006.01) C12P 21/08 (2006.01) G01N 33/577 (2006.01) G01N 33/86 (2006.01)
Patent
CA 2187670
The invention relates to a method for determining the antithrombin III (AT III) activated by a sulfated glycosaminoglycan or a sulfated oligosaccharide whose in vivo effect on AT III is similar to that of heparin, by an immuno-assay of the sandwich type in solid phase, characterized in that the method implements: a monoclonal antibody which recognizes, in solution, AT III less efficiently than AT III activated by a sulfated glycosaminoglycan or one of the sulfated oligosaccharides, having an activity on AT III similar to that of heparin; and a monoclonal antibody which substantially does not recognize native AT III in solution and AT III activated by one of said sulfated glycosaminoglycans or said oligosaccharides in solution, but which recognizes denaturated AT III.
L'invention concerne un procédé de détermination de l'antithrombine III (AT III) activée par un glycosaminoglycane sulfaté ou un oligosaccharide sulfaté dont l'effet in vivo sur l'AT III est analogue à celui de l'héparine, par un immunodosage de type sandwich en phase solide, caractérisé en ce qu'on met en oeuvre: - un anticorps monoclonal qui reconnaît en solution moins efficacement, l'AT III que l'AT III activée par un glycosaminoglycane sulfaté ou un des oligosaccharides sulfatés, ayant une activité sur l'AT III analogue à celle de l'héparine, et - un anticorps monoclonal qui ne reconnaît pratiquement pas l'AT III native en solution et l'AT III activée par l'un des dits glycosaminoglycanes sulfatés ou des dits oligosaccharides en solution mais qui reconnaît l'AT III dénaturée.
Lormeau Jean-Claude
Mille Blandine Georgette Renee
Paolucci Francis Egiste Joseph
Pau Bernard
Pasteur Sanofi Diagnostics
Robic
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