Method for the immunoanalysis of cholesterol epoxides

Details Method for the immunoanalysis of cholesterol epoxides Method for the immunoanalysis of cholesterol epoxides

C - Chemistry, Metallurgy

07

J

260/19, 167/37,

C07J 43/00 (2006.01) C07J 9/00 (2006.01) C07J 31/00 (2006.01) C07J 41/00 (2006.01) C07J 71/00 (2006.01) C07K 5/08 (2006.01) G01N 33/92 (2006.01)

Patent

CA 1250831

ABSTRACT An immunoassay is provided for cholesterol epoxide. To prepare the antibodies used in the immunoassay, novel immunogens, are prepared which comprise a 3,5(6)-trans- diaxial-dihydroxycholestane-6(5)-yl-hapten adduct linked to a covalently bonded bridge to a carrier protein. To detect cholesterol epoxide in the sample, it is converted to the hapten adduct, then contacted with the selected antibody.

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