Method for the isolation and purification of hepatitis b...

Details Method for the isolation and purification of hepatitis b... Method for the isolation and purification of hepatitis b...

A - Human Necessities

61

K

167/130

A61K 39/29 (2006.01) C07K 14/02 (2006.01)

Patent

CA 1259922

Improved method for the isolation and purification of hepatitis B surface antigen Abstract The supernatant of engineered yeast cells having produced hepatitis B surface antigen and disrupted in the presence of a polysorbate non-ionic detergent is clarified by addition of urea and adsorbed on colloïdal silica from which the purified antigen is desorbed with a low ionic strength buffer supplemented with urea and a polysorbate non-ionic detergent.

509934

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