Method for the isolation of expressed sequence tags in plants

Details Method for the isolation of expressed sequence tags in plants Method for the isolation of expressed sequence tags in plants

C - Chemistry, Metallurgy

12

N

C12N 15/10 (2006.01)

Patent

CA 2530304

A method is described for the isolation of Expressed Sequence Tags (ESTs) which are based on the use of suitable quantities of 5-azaCytidine in the germination phase of plants to induce the demethylation phenomenon of the DNA. In this way, it is possible to obtain "totipotent" seedlings, i.e. capable of expressing as many genes as possible regardless of the development phase of the plant and isolating said genes by the synthesis of a single cDNA library.

L'invention concerne un procédé d'isolation d'étiquettes de séquences exprimées (EST) fondé sur l'utilisation de quantités adéquates de 5-azacytidine dans la phase de germination de plantes pour induire la déméthylation de l'ADN. Ainsi, on peut obtenir des semis "totipotents", c'est-à-dire des semis pouvant exprimer autant de gènes que possible quelle que soit la phase de développement de la plante, et isoler lesdits gènes par synthèse d'une seule banque d'ADN complémentaires.

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