C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 5/00 (2006.01) C12N 5/06 (2006.01)
Patent
CA 2667073
The present invention provides a method of isolating a pluripotent cell from a pre-implantation embryo without isolation of the pluripotent cells from other cells, the method including propagating a whole pre-implantation embryo including one or more pluripotent cells, embedded in a feeder cell layer and cultivated in a medium substantially free of serum, and isolating a pluripotent cell from the one or more pluripotent cells. The present invention also provides pluripotent cells generated by the method and uses thereof.
La présente invention concerne un procédé destiné à isoler une cellule pluripotente à partir d'un embryon pré-implantatoire sans isoler les cellules pluripotentes des autres cellules, le procédé consistant à propager un embryon pré-implantatoire entier comprenant une ou plusieurs cellule(s) pluripotente(s), incorporé dans une couche de cellules nourricières et cultivé dans un milieu essentiellement exempt de sérum, et à isoler une cellule pluripotente parmi la ou les cellule(s) pluripotente(s). La présente invention concerne également des cellules pluripotentes générées par le procédé et leurs utilisations.
Nottle Mark Brenton
Vassiliev Ivan
Adelaide Research And Innovation Pty Ltd
Sim & Mcburney
LandOfFree
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