Method for the isolation of symbiotic microorganisms from...

Details Method for the isolation of symbiotic microorganisms from... Method for the isolation of symbiotic microorganisms from...

C - Chemistry, Metallurgy

12

N

195/44

C12N 1/00 (2006.01) A01N 59/16 (2006.01) C12Q 1/24 (2006.01)

Patent

CA 1159780

Abstract of the Disclosure Symbiotic microorganisms such as nitrogen fixing bacteria and phosphorus-providing fungi are isolated from nodules and mycorrhiza on the roots of host plants by sterilizing the surface and subsurface areas of root tissue with an aqueous solution of a fixative containing osmium tetraoxide. The osmium tetraoxide is highly soluble in water, and volatil- izes at room temperature to form a vapor which penetrates subsurface air pockets in root tissues to kill contaminating microorganisms. The osmium tetraoxide also provides a visual indicator of its progress through plant tissue by a progressive darkening of such tissue from the surface of root samples toward their centers. In this manner, the sterilization can be halted before the centrally located causitive bacteria is destroyed.

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