Method for the measurement of tsh

Details Method for the measurement of tsh Method for the measurement of tsh

G - Physics

01

N

167/37

G01N 33/74 (2006.01) G01N 33/543 (2006.01) G01N 33/577 (2006.01) G01N 33/76 (2006.01)

Patent

CA 1280691

Abstract: The invention provides an improved method for the measurement of TSH in blood or other body fluids by immunoassay such as EIA and RIA, particularly by the sandwich method. The method involves subjecting a test sample containing TSH (antigen) to an antigen- antibody reaction with an insolubilized antibody (the solid phase) and an enzyme- or radioisotope-labelled antibody, separating the labelled antibody bound to the antigen to be determined from the free labelled antibody, and measuring the amount of the bound antigen. In the method, an insolubilized TSH-.beta. subunit-specific monoclonal anti- body is used as the solid phase and a labelled TSH-.beta. subunit-specific monoclonal antibody is used as the labelled antibody, wherein the two TSH-.beta. subunit-specific monoclonal antibodies have different epitopes to be recognized. According to this method, the concentration of TSH can easily be measured with high sensitivity and high specificity within a very short period of time without inhibition caused by the simultaneous presence of other glycoprotein hormones, such as LH, HCG and FSH. The method is useful for the diagnosis of various diseases in which TSH participates, such as protopathic hypo- thyroidism, Bassedow's disease and cretinism.

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