C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/527 (2006.01) C12Q 1/02 (2006.01) G01N 33/50 (2006.01) G01N 33/573 (2006.01) G01N 33/574 (2006.01)
Patent
CA 2100835
- 21 - ABSTRACT OF THE DISCLOSURE The invention relates to a method for assessing clinical activity and resistance to multiple anticancer drugs of mammalian cells, based on a dose-dependent inhibition with cytotoxic agents of the cellular rise in ornithine decarboxylase activity, following serum stimulation, which comprises the steps of: (d) determining by an immu- nological technique the rise in the ornithine decarboxy- lase protein levels, after adding serum containing medium to quiescent mammalian cells, and (b) obtaining the levels of the anticancer drug resistance from the ratio of 50% inhibitory concentration of the ornithine decarboxyla- se obtained with anticancer drug resistance cells and drug sensitive cells. The immunological technique is selected from ELISA, radioimmunoassay,immunofluorescence, flow cytometry and fluorescence microscopy. The increase in the ornithine decarboxylase activity resulted from an increase in the number of ornithine decarboxylase molecules.
Assaraf Yehuda G.
Bachrach Uriel
Sim & Mcburney
Technion Research & Development Foundation Ltd.
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