Method for the permanent expression of glutamate receptors

Details Method for the permanent expression of glutamate receptors Method for the permanent expression of glutamate receptors

C - Chemistry, Metallurgy

12

N

C12N 15/12 (2006.01) C07K 14/705 (2006.01) C12N 5/10 (2006.01) G01N 33/566 (2006.01)

Patent

CA 2186788

The invention concerns a method for the preparation of eucaryotic cell lines for the permanently ectopic expression of glutamate receptors, the cell lines being prepared by the transformation of cells containing nucleic acids coding for glutamate receptors. The method is characterized in that, during the establishment of the cell lines, at least one of the following conditions is satisfied for the culture: a) the transformed cells are cultured in a culture medium containing a glutamate precursor; b) the transformed cells are cultured in the presence of a glutamate-receptor antagonist: c) the transformed cells ant cultured in a first phase under conditions in which glutamate-receptor expression is repressed and in a second phase under conditions in which the repression is discontinued. The invention also concerns the cell lines thus obtained and their use.

L'invention concerne un procédé de préparation de lignées cellulaires eucaryotes pour l'expression ectopique permanente de récepteurs du glutamate, par transformation des cellules avec des acides nucléiques codant pour des récepteurs du glutamate, caractérisé en ce que, lors de l'établissement des lignées cellulaires, on observe au moins l'une des conditions de culture ci-après: a) culture des cellules transformées dans un milieu de culture renfermant un précurseur du glutamate; b) culture des cellules transformées en présence d'un antagoniste du récepteur du glutamate; c) culture des cellules transformées dans une première phase, dans les conditions pour lesquelles l'expression du récepteur du glutamate est réprimée et, dans une seconde phase, dans les conditions pour lesquelles la répression est de nouveau interrompue. L'invention concerne également les lignées cellulaires obtenues et leur utilisation.

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