C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01)
Patent
CA 2310384
The invention relates to a method for characterising, classifying and distinguishing tissues and cell types, for predicting the behaviour of tissues and groups of cells, and for identifying genes which have altered in their expression. Said method is characterised in that the base cytosine (not 5-methyl-cytosine) in a genomic DNA taken from any tissue sample is converted into uracil through treatment with a bisulphite solution. Fractions of the genomic DNA which has been treated are amplified by using very short or degenerated oligonucleotides, and the remaining cytosines of the amplified fractions are detected by means of hybridisation or polymerase reaction. The data generated from the analysis and automatically transferred to a processing algorithm is then used to draw conclusions as to the phenotype of the cell material which was analysed.
L'invention concerne un procédé de caractérisation, de classification et de différenciation de tissus et de types de cellules, permettant de prévoir le comportement de tissus et groupes de cellules et d'identifier des gènes d'expression modifiée. Ce procédé est caractérisé en ce que, dans un ADN génomique extrait d'un tissu quelconque, la base cytosine et non la 5-méthylcytosine est transformée par traitement avec une solution bisulfite dans de l'uracile. Des fractions de l'ADN génomique ainsi traité sont amplifiées par utilisation d'oligonucléotides très courts ou dégénérés et la cytosine restante des fractions amplifiées est détectée par hybridation ou réaction polymérase de telle façon que les données, générées lors d'une telle analyse et transmises automatiquement à un algorithme de traitement, permettent de déduire le phénotype du matériau cellulaire analysé.
Olek Alexander
Olek Sven Stefan
Walter Jorn
Epigenomics Aktiengesellschaft
Epigenomics Gmbh
Macrae & Co.
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