Method for the preparation of glucosyltransferase

Details Method for the preparation of glucosyltransferase Method for the preparation of glucosyltransferase

C - Chemistry, Metallurgy

12

N

195/123

C12N 9/10 (2006.01)

Patent

CA 1183477

METHOD FOR THE PREPARATION OF GLUCOSYLTRANSFERASE ABSTRACT OF THE DISCLOSURE A method of preparing a purified glucosyltransferase (GTF) for use in immunization against dental caries, which method comprises: culturing a Streptococcus mutans in a medium contain- ing glucose and dialyzable nutrients to form a mixture of culture cells and supernatant; recovering the supernatant by the removal of the culture cells; admixing the recovered supernatant with a water-insoluble, polymerized polysaccharide as solid particulate material for a period of time, to provide a GTF, solid particulate complex; recovering the GTF complex in solid particulate form by filtration; washing the solid GTF complex to remove unbound GTF and medium component; removing the GTF from the solid particulate material by a denaturing solvent; recovering the water-insoluble particulate material for reuse in the method; and recovering the GTF from the water-insoluble polysaccharide and purifying the recovered GTF. -1-

420426

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