Method for the preparation of optical (bio)chemical sensor...

B - Operations – Transporting – 01 – L

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Details

B01L 3/00 (2006.01) B01J 19/00 (2006.01) B01L 3/02 (2006.01) G01N 21/55 (2006.01)

Patent

CA 2438345

The present invention relates to a method for the preparation of a miniaturized optical chemical or biochemical sensor device (e.g. bulk optode, etc. for ion sensing), said device comprising a substrate material having a planar surface portion, said planar surface representing a transducer based on an optical phenomenon such as surface plasmon resonance based on evanescent waves, reflection or transmission; said planar surface portions having arranged thereon an multi-analyte array of (bio)chemical sensor dots located at spatially separated predetermined positions of the planar surface, said sensor dots including (i) a polymer matrix, and (ii) on or more (bio)chemical recognition moieties, the method comprising (a) providing a substrate material having a planar surface portion; (b) providing one or more spotting fluid(s); (c) depositing the one or more spotting fluid(s) onto the planar surface portion of the substrate material by means of a pin-printer depositing mechanism (arrayer) and allowing the spotting fluid(s) to consolidate.

L'invention concerne un procédé de fabrication d'un dispositif de détection optique chimique ou biochimique miniature (p. ex. optode globale pour la détection ionique). Le dispositif comprend un matériau substrat doté d'une partie de surface plane, celle-ci représentant un transducteur basé sur un phénomène optique, tel que la résonance plasmonique de surface basée sur des ondes évanescentes, la réflexion ou la transmission. La partie de surface plane présente un réseau multi-analyte de points de détection (bio)chimiques situés à des positions séparées dans l'espace prédéterminées de la surface plane, les points de détection comprenant (i) une matrice polymère et (ii) une ou plusieurs fractions de reconnaissance (bio)chimiques. Le procédé consiste a) à fournir un matériau substrat comportant une partie de surface plane, b) à fournir un ou plusieurs fluide(s) de tachage, c) à déposer le(s) fluide(s) de tachage sur la partie de surface plane du matériau substrat au moyen d'un mécanisme de dépôt d'une imprimante à aiguilles et à laisser les fluides de tachage se solidifier.

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