Method for the production of 1,3-propanediol by recombinant...

C - Chemistry – Metallurgy – 12 – P

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C12P 7/18 (2006.01) C07K 14/24 (2006.01) C12N 1/21 (2006.01) C12N 9/04 (2006.01) C12N 9/16 (2006.01) C12N 9/88 (2006.01) C12N 15/52 (2006.01) C12N 15/53 (2006.01) C12N 15/55 (2006.01) C12N 15/60 (2006.01)

Patent

CA 2326722

Recombinant organisms are provided comprising genes encoding genes encoding glycerol dehydratase, 1,3-propanediol oxidoreductase, a gene encoding vitamin B12 receptor precursor (BtuB), a gene encoding vitamin B12 transport system permease protein (BtuC) and a gene encoding vitamin B12 transport ATP-binding protein (BtuD). The recombinant microorganism is contacted with a carbon substrate and 1,3-propanediol is isolated from the growth media.

L'invention concerne des organismes de recombinaison comprenant des gènes codant des gènes codant glycérol déhydratase, 1,3-propanediol oxydoréductase, un gène codant le précurseur du récepteur de la vitamine B¿12? (BtuB), un gène codant la protéine de perméase du système de transport de la vitamine B¿12? (BtuC) et un gène codant la protéine de liaison à ATP jouant un rôle dans le transport de la vitamine B¿12? (BtuD). On met en contact le micro-organisme de recombinaison avec un substrat de carbone et on isole 1,3-propanediol du milieu de croissance.

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