Method for the production of .gamma.-linolenic acid and/or...

Details Method for the production of .gamma.-linolenic acid and/or... Method for the production of .gamma.-linolenic acid and/or...

C - Chemistry, Metallurgy

12

N

C12N 15/82 (2006.01) C07K 14/415 (2006.01)

Patent

CA 2625505

The invention relates to the production of .gamma.-linolenic acid (18:3 .DELTA.6,9,12) or stearidonic acid (18:4 .DELTA.6,9,12,15) or .gamma.- linolenic acid (18:3.DELTA.6,9,12) and stearidonic acid (18:4 .DELTA.6,9,12,15) in transgenic plants of the brassicaceae family, said transgenic plants containing at least 10 percent by weight of oleic acid relative to the total fatty acid concentration and being provided with an increased .DELTA.-6-C18 fatty acid concentration as a result of the activity of the .DELTA.-6 desaturases used in the method. The invention also relates to novel nucleic acid sequences coding for the .DELTA.-6 desaturases used in the method, gene constructs containing said nucleic acid sequences, a vector and transgenic plants containing at least one nucleic acid sequence or a gene construct.

L'invention concerne la production d'acide ?-linolénique (18:3?6,9,12) ou d'acide stéaridonique (18:4 ?6,9,12,15) ou d'acide ?-linolénique (18:3 ?6,9,12) et d'acide stéaridonique (18:4 ?6,9,12,15) dans des plantes transgéniques de la famille des Brassicacées, les plantes transgéniques contenant au moins 10 % en poids d'acide oléique par rapport à la teneur totale en acides gras et présentant une teneur accrue en acides gras C18 ?6 en raison de l'activité des ?6-désaturases utilisées dans le procédé. L'invention concerne également de nouvelles séquences d'acides nucléiques codant pour les ?6-désaturases utilisées dans le procédé, des gènes chimères contenant ces séquences d'acides nucléiques, un vecteur et des plantes transgéniques contenant au moins une telle séquence d'acides nucléiques ou un tel gène chimère.

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