Method for the production of immobilized glucose isomerase

C - Chemistry – Metallurgy – 12 – N

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C12N 11/02 (2006.01) C12N 9/92 (2006.01) C12N 11/00 (2006.01) C12N 11/08 (2006.01) C12N 11/12 (2006.01) C12N 11/14 (2006.01) C12P 19/24 (2006.01)

Patent

CA 1159785

3231 ABSTRACT OF THE DISCLOSURE An immobilized glucose isomerase is prepared by adding a non-ionic surfactant to a cultured material. This cultured material is obtained by culturing a glucose isomerase producing microorganism. The cultured material is then autolysed in order to solubilize the glucose iso- merase without solubilizing the polysaccharides present. A glucose isomerase solution containing no or almost no polysaccharides is then separated. Said glucose isomerase solution is then contacted with a carrier which is capable of adsorbing glucose isomerase. WRR/SV/1B-25

369911

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