Method for the production of insulin-like growth factor-i

Details Method for the production of insulin-like growth factor-i Method for the production of insulin-like growth factor-i

C - Chemistry, Metallurgy

07

K

C07K 14/65 (2006.01) C12N 15/12 (2006.01) C12N 15/62 (2006.01) A61K 47/48 (2006.01) C12N 9/52 (2006.01)

Patent

CA 2658736

Method for the production of IGF-I, characterized by cultivating a prokaryotic host cell comprising an expression vector containing a nucleic acid encoding a fusion protein comprising said IGF-I N-terminally linked to the C-terminus of a propeptide, whereby said propeptide ends C-terminally with amino acids -Y-Pro, wherein Y is selected from the group consisting of Pro, Pro-Ala, Pro-Gly, Pro-Thr, Ala-Pro, Gly-Pro, Thr-Pro, Arg-Pro, or Pro-Arg-Pro, recovering and cleaving said fusion protein with IgA protease, and recovering said IGF-I. IGF-I is useful for the treatment of neurodegenerative disorders like Alzheimer's Disease.

La présente invention concerne un procédé de production de IGF-1, caractérisé par la culture d'une cellule procaryote hôte comprenant un vecteur d'expression qui contient un acide nucléique codant pour une protéine de fusion comprenant ledit IGF-1 lié par son N-terminal au C-terminal d'un propeptide, ledit propeptide se terminant à son C-terminal par des acides aminés -Y-Pro, ledit Y étant choisi dans le groupe comprenant Pro, Pro-Ala, Pro-Gly, Pro-Thr, Ala-Pro, Gly-Pro, Thr-Pro, Arg-Pro et Pro-Arg-Pro, par la récupération et le clivage de ladite protéine de fusion à l'aide d'IgA protéase, et par la récupération dudit IGF-1. L'IGF-1 est utilisable pour le traitement d'affections neurodégénératives telles que la maladie d'Alzheimer.

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