Method for the production of neutral protease

C - Chemistry – Metallurgy – 12 – N

Patent

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195/1.14, 195/1.

C12N 15/00 (2006.01) C12N 9/50 (2006.01) C12N 9/56 (2006.01) C12N 15/75 (2006.01)

Patent

CA 1285233

ABSTRACT Method for the production of neutral protease, for industrial uses such as for example in the food and chemical industries, and particularly in detergents, comprising: a) constructing a hybrid recombinant plasmid containing the gene which codes for the neutral protease; b) isolating a mutant strain of Bacillus subtilis; c) introducing the recombinant hybrid plasmid into the mutant strain; d) isolating the clones containing the hybrid plasmid; e) cultivating the clones in a liquid culture medium, under aerobic conditions; and f) isolating the neutral protease obtained from the culture medium.

492088

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