Method for the production of non-group c adenoviral vectors

Details Method for the production of non-group c adenoviral vectors Method for the production of non-group c adenoviral vectors

C - Chemistry, Metallurgy

12

N

C12N 15/861 (2006.01) A61K 48/00 (2006.01) C12N 5/10 (2006.01) C12N 7/00 (2006.01) C12N 15/86 (2006.01)

Patent

CA 2289611

A method of producing a replication deficient adenovirus comprising a passenger gene and a deficiency in an essential gene function of the E1 region of an adenovirus comprising producing the adenovirus in a cell that provides in trans gene functions of the E1 and E4 regions of one or more adenoviruses not belonging to the same serogroup as the replication deficient adenovirus.

Cette invention se rapporte à un procédé de production d'un adénovirus inapte à la réplication, comportant un gène passager et présentant une insuffisance de fonction de gène essentiel de la région E1 d'un adénovirus. Ledit procédé consiste à produire l'adénovirus dans une cellule qui assure des fonctions géniques in trans des régions E1 et E4 d'un ou de plusieurs adénovirus n'appartenant pas au même sérogroupe que l'adénovirus inapte à la réplication.

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