Method for the production of permanent human cell lines

Details Method for the production of permanent human cell lines Method for the production of permanent human cell lines

C - Chemistry, Metallurgy

12

N

C12N 15/00 (2006.01) C12N 5/10 (2006.01) C12N 15/85 (2006.01) C12N 15/86 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2629211

The invention relates to a method for producing a permanent human cell lineage, in which isolated primary human cells are simultaneously transfected with a sequence allowing at least one cell-transforming factor to be expressed and a sequence allowing at least one recombinant polypeptide to be expressed.

La présente invention concerne un procédé de production d'une lignée cellulaire humaine, procédé selon lequel des cellules humaines primaires isolées sont transfectées en même temps qu'une séquence permettant l'expression d'au moins un facteur transformant de ces cellules et qu'une séquence permettant l'expression d'au moins un polypeptide recombinant.

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