Method for the production of polyfructans

Details Method for the production of polyfructans Method for the production of polyfructans

C - Chemistry, Metallurgy

12

N

C12N 15/54 (2006.01) A01H 1/00 (2006.01) A01H 5/00 (2006.01) A01H 5/08 (2006.01) A01H 5/10 (2006.01) A23L 1/29 (2006.01) C07H 3/06 (2006.01) C07K 16/16 (2006.01) C07K 16/40 (2006.01) C12N 1/19 (2006.01) C12N 1/21 (2006.01) C12N 5/04 (2006.01) C12N 5/10 (2006.01) C12N 9/10 (2006.01) C12N 15/11 (2006.01) C12N 15/63 (2006.01) C12N 15/82 (2006.01) C12P 19/00 (2006.01) C12P 19/04 (2006.01) C12P 19/12 (2006.01) C12P 19/14 (2006.01) C12P 19/18 (2006.01)

Patent

CA 2431994

The invention relates to nucleic acids coding for modified polypeptides with fructosyltransferase activity, the vectors containing said nucleic acids for the expression of the modified fructosyltransferase in prokaryotic or eukaryotic cells, the host cells or transgenic plants containing said vectors. The invention also relates to a method for the production of high-molecular polyfructans with mainly .beta.-1, 2 bonds and a very low degree of branching, especially of inulin. The invention further relates to a method for the production of fructooligosaccharides using the inulin produced according to the invention or using saccharose and to a method for the production of difructose dianhydrides using the inulin produced according to the invention or saccharose. The invention additionally relates to the utilization of the inulin produced according to the invention in the production of inulin ethers and inulin esters and to the utilization of fructooligosaccharides or hydrogenated fructooligosaccharides and difructose dianhydrides as food additive, especially as diet food product.

L'invention concerne des acides nucléiques qui sont codants pour les polypeptides modifiés ayant une activité de fructosyltransférase ; des vecteurs contenant ces acides nucléiques et destinés à l'expression de la fructosyltransférase modifiée dans ces cellules procaryotes ou eucaryotes ; les cellules hôtes contenant ces vecteurs et/ou les plantes transgéniques ; un procédé de production de polyfructosanes de masses moléculaires élevées et ayant principalement des liaisons .beta.béta-1, 2 et un très faible taux de ramification, notamment de l'insuline ; procédé de production des fructo-oligosaccharides à l'aide de l'inuline obtenue selon l'invention ou à l'aide de saccharose, ainsi qu'un procédé de production de dianhydrides de difructose à l'aide de l'inuline obtenue selon l'invention ou à l'aide de saccharose, ainsi que l'utilisation de l'inuline obtenue selon l'invention pour produire des éthers et esters d'inuline et l'utilisation de fructo-oligosaccharides ou de fructo-oligosaccharides hydratés et de dianhydrides de difructose comme additifs alimentaires, notamment comme denrée alimentaire diététique.

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