Method for the purification of albumin conjugates

Details Method for the purification of albumin conjugates Method for the purification of albumin conjugates

C - Chemistry, Metallurgy

07

K

C07K 19/00 (2006.01) A61K 47/42 (2006.01) A61K 47/48 (2006.01) B01D 15/08 (2006.01) C07K 1/20 (2006.01) C07K 14/76 (2006.01)

Patent

CA 2564031

A method for separating albumin conjugates from unconjugated albumin in a solution comprising albumin conjugates and unconjugated albumin by hydrophobic Interaction Chromatography (HIC). The solution is loaded onto the hydrophobic column equilibrated in aqueous buffer having a high salt content; applying to the column a gradient of decreasing salt concentration; and collecting the eluted albumin conjugates.

L'invention concerne un procédé qui permet de séparer des conjugués d'albumine d'albumine non conjuguée dans une solution comprenant des conjugués d'albumine et de l'albumine non conjuguée par chromatographie hydrophobe (HIC). Ce procédé consiste : à charger la solution sur la colonne hydrophobe équilibrée dans un tampon aqueux présentant une teneur élevée en sel ; à appliquer sur la colonne un gradient de concentration en sel décroissante ; et à recueillir les conjugués d'albumine élués.

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