Method for the purification of alpha-1-antitrypsin

A - Human Necessities – 61 – K

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A61K 38/57 (2006.01) C07K 1/22 (2006.01) C07K 14/81 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2631524

The invention relates to methods for the isolation of AAT from solutions containing albumin and AAT using at least two separate metal chelate chromatography steps. The product may be further purified and/or subjected to one or more virus inactivation or reduction steps. The isolated AAT may then be formulated for pharmaceutical use.

L~invention concerne des procédés d~isolation d~AAT à partir de solutions qui contiennent de l~albumine et de l~AAT en utilisant au moins deux étapes séparées de chromatographie à chélates métalliques. Le produit peut être davantage purifié et/ou soumis à une ou plusieurs étapes d~inactivation ou de réduction du virus. L~AAT isolée peut alors être formulée pour une utilisation pharmaceutique.

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