C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
195/71, 195/33.1
C12N 9/02 (2006.01) C12N 9/04 (2006.01)
Patent
CA 1182412
-1- METHOD FOR THE PURIFICATION OF CHOLESTEROL OXIDASE Abstract of the Disclosure A method for the purification and recovery of cholesterol oxidase is disclosed. Cholesterol oxidase is purified by a method including the steps of: (a) contacting a solution of the cholesterol oxidase to be purified with a chromatography composition comprising an unbound water-insoluble steroid which is a nonsubstrate for cholesterol oxidase so as to adsorb the cholesterol oxidase on the steroid; (b) separating the solution from the steroid; and (c) contacting the steroid with an eluting solution contacting a surfactant at a concentration between 0.001 per cent and 10 per cent so as to recover the adsorbed cholesterol oxidase. In a preferred embodiment, the solution containing the cholesterol oxidase to be purified is a microor- gansim growth medium having cholesterol oxidase activity and, prior to the step of contacting the solution with a steroid, the solution is contacted with an ion-exchange chromatography material. In yet another embodiment, the eluting solution con- taining the surfactant also contains a metal salt of a concentration of at least about 0.005 molar. There is also disclosed a method for forming a packed bed of a composition comprising the unbound steroid which comprises the step of first suspending the steroid which comprises the step of first suspending the steroid in a water-miscible organic solvent.
410802
Goodhue Charles T.
Snoke Roy E.
Eastman Kodak Company
Gowling Lafleur Henderson Llp
LandOfFree
Method for the purification of cholesterol oxidase does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for the purification of cholesterol oxidase, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for the purification of cholesterol oxidase will most certainly appreciate the feedback.
Profile ID: LFCA-PAI-O-1198534