Method for the purification of intact, correctly-folded...

Details Method for the purification of intact, correctly-folded... Method for the purification of intact, correctly-folded...

C - Chemistry, Metallurgy

12

N

C12N 15/18 (2006.01) C07K 1/14 (2006.01) C07K 1/20 (2006.01) C07K 14/65 (2006.01)

Patent

CA 2098183

Method for the recovery and purification of intact, correctly-folded, monomeric insulin-like growth factor-1 peptide from large volumes of 1GF-1-containing medium are described, comprising a series of adsorption-desorption steps employing a combination of cation exchange and hydrophobic interaction adsorbents. Product IGF-1 peptide is highly purified and suitable for use in a variety of clinical applications.

On décrit un procédé de purification et de récupération de peptides du facteur de croissance-1 analogue à l'insuline, (IGF-1), sous une forme intacte, monomère et correctement pliée, à partir de volumes importants d'un milieu contenant l'IGF-1. Ce procédé consiste à effectuer une série d'étapes d'adsorption de désorption au moyen d'une combinaison d'adsorbants d'échange cationique et d'interaction hydrophobe. Le peptide d'IGF-1 produit est fortement purifié et convient à toute une variété d'applications cliniques.

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