Method for the purification of leucocytosis-promoting factor...

C - Chemistry – Metallurgy – 07 – K

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167/139, 195/78

C07K 14/235 (2006.01) A61K 35/66 (2006.01) B01D 15/00 (2006.01) A61K 39/00 (2006.01)

Patent

CA 1210720

- 1 - Abstract The invention provides a method for producing highly pure LPF-HA (Leucocytosis-promoting Factor Hemagglutinin) from the culture media of Bordetella pertussis in a high yield by an affinity chromatography using a denatured ceruloplasmin as a ligand. The LPF-HA obtained by this invention is highly pure and does not contain endotoxins of B. pertussis and is useful for the preparation of various reagents utilizing its physiological activities, as a medicine for treating diabetes, and as a pertussis vaccine.

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