Method for the purification of proteins

Details Method for the purification of proteins Method for the purification of proteins

C - Chemistry, Metallurgy

12

N

530/3.2, 195/1.2

C12N 9/64 (2006.01) C07K 14/745 (2006.01)

Patent

CA 1314011

X-7029A Abstract of the Disclosure The recovery of vitamin X-dependent proteins produced by transformed microorganisms can be effected from the cell culture medium utilizing the changes in the protein which occur in the presence of divalent cations. The present process uses divalent cations to alter the binding affinity of the proteins and thereby selectively elute the proteins away from contaminants in the culture medium using standard chromatography.

613553

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