Method for the purification of proteins

C - Chemistry – Metallurgy – 07 – K

Patent

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Details

C07K 1/22 (2006.01) C07K 14/555 (2006.01) C07K 14/56 (2006.01) C12N 9/26 (2006.01) C12N 9/28 (2006.01) C12N 9/76 (2006.01)

Patent

CA 2226080

The instant invention relates to a new method for the purification of proteins using copper chelate-affinity chromatography, wherein the impure or pre- purified protein is adsorbed on immobilized copper(II) ions, optionally washed with buffer and deionized water, washed with a solution of a Lewis-base, and finally eluted with deionized water.

L'invention porte sur un nouveau procédé de purification de protéines par chromatographie à affinité cuivre/chélate dans lequel les protéines impures ou avant purification sont adsorbées sur des ions immobilisés de cuivre (II) éventuellement lavés à l'aide de tampons et d'eau désionisée, lavés à l'aide d'une solution d'une base de Lewis, et finalement éluées à l'aide d'eau désionisée.

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